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Fig. 3 | Cardiovascular Diabetology

Fig. 3

From: TNF-α induces vascular insulin resistance via positive modulation of PTEN and decreased Akt/eNOS/NO signaling in high fat diet-fed mice

Fig. 3

Vascular PTEN protein phosphorylation modulates insulin-induced relaxation in HFD-fed mice. Western blot quantification of total (a) and phosphorylated (b) PTEN expression levels in mesenteric arteries. Concentration-effect curves to insulin were performed in endothelium-intact resistance mesenteric arteries. The role of PTEN in the vasculature was investigated using VO-OHpic in vessels of C57Bl/6J mice fed with control and HFD diets (c) and vessels of C57Bl/6J incubated with TNF-α (d). Western blot quantification of phosphorylated PTEN expression levels in mesenteric arteries (e). Results represent the mean ± S.E.M. n = 5–6 in each experimental group. *p < 0.05 vs. C57Bl/6J Control; #p < 0.05 vs. C57Bl/6J HFD; &p < 0.05 vs. C57Bl/6J Control_TNF-α

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Cardiovascular Diabetology

ISSN: 1475-2840