Apocynin influence on oxidative stress and cardiac remodeling of spontaneously hypertensive rats with diabetes mellitus

Table 5 Isolated papillary muscle data after positive inotropic stimulation

		SHR (n = 13)	SHR-APO (n = 11)	SHR-DM (n = 11)	SHR-DM-APO (n = 10)
PP30	DT	13.7 ± 3.09	11.1 ± 1.69*	11.9 ± 3.01	10.3 ± 2.87
	RT	1.04 ± 0.30	1.06 ± 0.31	1.24 ± 0.52	1.25 ± 0.62
	+dT/dt	140 ± 33.4	115 ± 23.1*	105 ± 25.0*	95.2 ± 27.8
	TPT	215 ± 17.1	209 ± 13.0	255 ± 27.0*	250 ± 22.6^#
	−dT/dt	42.6 ± 9.67	35.8 ± 4.58	32.4 ± 8.73*	25.1 ± 8.45^#§
2.5 mM [Ca⁺²]₀	DT	12.5 ± 2.78	10.3 ± 1.49	11.0 ± 2.95	9.79 ± 2.85
	RT	0.87 ± 0.30	0.90 ± 0.31	0.90 ± 0.34	0.92 ± 0.52
	+dT/dt	140 ± 31.9	117 ± 21.4	108 ± 28.0*	97.9 ± 28.7
	TPT	192 ± 16.9	186 ± 17.8	224 ± 23.2*	216 ± 15.1^#
	−dT/dt	48.3 ± 10.6	40.0 ± 6.27	38.4 ± 11.6*	30.7 ± 10.2^#
Iso 10⁻⁶ M	DT	11.4 (8.33–13.5)	9.70 (8.77–10.1)	9.68 (8.60–10.1)	9.47 (6.08–10.8)
	RT	0.85 ± 0.43	0.86 ± 0.34	0.84 ± 0.28	0.83 ± 0.52
	+dT/dt	136 ± 34.0	118 ± 22.4	96.9 ± 26.6*	96.7 ± 31.7
	TPT	163 ± 11.1	157 ± 9.49	184 ± 16.9*	190 ± 15.8^#
	−dT/dt	67.9 ± 16.6	58.3 ± 8.03	46.4 ± 13.4*	39.1 ± 13.7^#

Data are expressed as mean ± standard deviation or median and 25th and 75th percentile
SHR spontaneously hypertensive rats; SHR-APO SHR treated with apocynin; SHR-DM diabetic SHR; SHR-DM-APO diabetic SHR treated with apocynin; PP30 30 s post-rest contraction; 2.5 mM [Ca ⁺² ] ₀ extracellular Ca²⁺ concentration increased to 2.5 mM; Iso 10 ⁻⁶ M β-adrenergic agonist isoproterenol (10⁻⁶ M) added to the nutrient solution; DT peak of developed tension (g/mm²); RT resting tension (g/mm²); +dT/dt maximum rate of tension development (g/mm²/s); TPT time to peak tension (ms); −dT/dt maximum rate of tension decline (g/mm²/s)
Two-factor ANOVA; * p < 0.05 vs SHR; ^# p < 0.05 vs SHR-APO; ^§ p < 0.05 vs SHR-DM

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