Fig. 5
From: Glucose intolerance after chronic stress is related with downregulated PPAR-γ in adipose tissue
Expressome and Morphologic analyses of the white adipose tissue. The expression of Lcn2 mRNA quantified by real-time PCR was significantly increased in animals submitted to CUS in the white adipose tissue and returned to basal levels after the recovery period (a). No differences were found in the expression of TNF-α, IL-1, Mcp-1, Cxcl-1 or Cxcl-10 in vWAT (b–g). h shows the immunohistochemistry of vWAT. It revealed the presence of Lcn2 positive mononuclear inflammatory cells in CUS-submitted animals (h), mainly composed by macrophages and monocytes (inset); and also the presence of Lcn2 protein-positive inflammatory cells in CUS group after stress (red arrow). These findings reverted after recovery. CUS chronic unpredictable stress; Cxcl chemokine (C-X-C motif) ligand; IL interleukin; Lcn2 lipocalin-2; LPL lipoprotein lipase; Mcp-1 monocyte chemoattractant protein-1; TNF-α tumor necrosis factor-α; *p < 0.05; **p < 0.01