Fig. 5

Western blot analysis of Bax, Bcl-2, pro caspase-3 and caspase-3 p17 proteins in vascular endothelial cells. a Total bax protein expression in vascular endothelial cells. b Mitochondrial Bax protein expression in vascular endothelial cells. c Bcl-2 expression in vascular endothelial cells. d Pro caspase-3 and caspase-3 p17 expression in vascular endothelial cells. SAL (SAL group rats underwent 48 h infusions with saline); CHG (CHG group rats underwent 48 h infusions with 50 % glucose continually to keep the glucose concentration at 20 ± 0.5 mmol/l); AFG (AFG group rats underwent 48 h infusions with 50 % glucose intermittently so that blood glucose alternated between 5.5 ± 0.5 and 20 ± 0.5 mmol/l). β-Actin antibodies were used as loading controls. A representative western blot is shown. Data are mean ± SD. n = 10/group. *P < 0.05 vs SAL; ^#P < 0.05 vs CHG