Table 3 Confirmation by qRT-PCR
From: Transcriptomic alterations in the heart of non-obese type 2 diabetic Goto-Kakizaki rats
Gene symbol | Gene name | Acc. nr. | DNA microarray | qRT-PCR | Confirmed | ||
|---|---|---|---|---|---|---|---|
AVE (log2) | Fold change | Fold change | SEM | ||||
Adipoq | Adiponectin, C1Q and collagen domain containing | Rn00595250_m1 | −4.99 | −31.78 | −23.64 | 0.02 | Yes |
Retn | Resistin | Rn00595224_m1 | −4.74 | −26.72 | 31.08 | 14.46 | No |
Atp1b4 | ATPase, (Na+)/K+ transporting, beta 4 polypeptide | Rn00584523_m1 | −4.21 | −18.51 | −4.74 | 0.05 | Yes |
Car3 | Carbonic anhydrase 3 | Rn01461970_m1 | −3.73 | −13.27 | −7.88 | 0.03 | Yes |
Cma1 | Chymase 1, mast cell | Rn00565319_m1 | −3.11 | −8.63 | −5.12 | 0.02 | Yes |
Arntl | Aryl hydrocarbon receptor nuclear translocator-like | Rn00577590_m1 | −2.75 | −6.74 | −2.95 | 0.03 | Yes |
Tgm1 | Transglutaminase 1, K polypeptide | Rn00581408_m1 | −1.97 | −3.92 | −4.88 | 0.03 | Yes |
Nnat | Neuronatin (Nnat), transcript variant 1 | Rn00440480_m1 | −1.87 | −3.66 | −4.2 | 0.02 | Yes |
Ddah1 | Dimethylarginine dimethylaminohydrolase 1 | Rn00574200_m1 | −1.69 | −3.23 | −3.36 | 0.02 | Yes |
Ntrk3 | Neurotrophic tyrosine kinase, receptor, type 3 | Rn00570389_m1 | −1.69 | −3.23 | −2.33 | 0.06 | Yes |
Stat3 | Signal transducer and activator of transcription 3 | Rn00562562_m1 | −1.49 | −2.81 | 1.36 | 0.08 | No |
Dpp4 | Dipeptidylpeptidase 4 | Rn00562910_m1 | −1.35 | −2.55 | −2.11 | 0.03 | Yes |
Ephx2 | Epoxide hydrolase 2, cytoplasmic | Rn00576023_m1 | −1.33 | −2.51 | −4.23 | 0.02 | Yes |
Gpc3 | Glypican 3 | Rn00516722_m1 | −1.29 | −2.45 | −2.54 | 0.04 | Yes |
Fgf18 | Fibroblast growth factor 18 | Rn00433286_m1 | −1.17 | −2.25 | −2.36 | 0.03 | Yes |
Tfpi | Tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor) | Rn00567935_m1 | −1.10 | −2.14 | 1.12 | 0.06 | No |
Gckr | Glucokinase (hexokinase 4) regulator | Rn00565467_m1 | −0.98 | −1.97 | −2.09 | 0.05 | N/A |
Cdkn1a | Cyclin-dependent kinase inhibitor 1A | Rn00589996_m1 | −0.93 | −1.91 | −1.34 | 0.04 | N/A |
Bat5 | HLA-B associated transcript 5 | Rn01525709_g1 | 0.96 | 1.95 | −1.3 | 0.06 | N/A |
Sele | Selectin E | Rn00594072_m1 | 1.02 | 2.03 | 2.35 | 0.14 | Yes |
Dbp | D site of albumin promoter (albumin D-box) binding protein | Rn00497539_m1 | 1.06 | 2.08 | 2.39 | 0.17 | Yes |
Abcg1 | ATP-binding cassette, subfamily G (WHITE), member 1 | Rn00585262_m1 | 1.08 | 2.11 | 1.99 | 0.14 | No |
Cyr61 | Cysteine-rich, angiogenic inducer, 61 | Rn00580055_m1 | 1.14 | 2.20 | 1.58 | 0.12 | No |
Ephx1 | Epoxide hydrolase 1, microsomal | Rn00563349_m1 | 1.21 | 2.31 | 2.24 | 0.18 | Yes |
Prkce | Protein kinase C, epsilon | Rn01769089_m1 | 1.28 | 2.43 | 1.09 | 0.08 | No |
Nurp1 | Nuclear protein, transcriptional regulator, 1 | Rn00586046_m1 | 1.34 | 2.53 | 3.29 | 0.28 | Yes |
Slc16a7 | Solute carrier family 16, member 7 (monocarboxylic acid transporter 2) | Rn00568872_m1 | 1.77 | 3.41 | 3.21 | 0.19 | Yes |
Atp2b2 | ATPase, C++Â transporting, plasma membrane 2 | Rn01425460_m1 | 1.88 | 3.68 | 3.78 | 0.21 | Yes |