Fig. 4

Effect of insulin glargine and insulin degludec on the beating-rate of human iPSC-derived cardiomyocytes. Commercially available human iPSC-derived cardiomyocytes (Cor.4U^®) were used to assess the long-term effect of insulins on the beating-rate (a–d). Experiments were performed at day 4 after seeding. Cells were treated with 500 nM of insulin or insulin analogues or 100 nM isoproterenol, respectively. The beating-rate was measured for 6 h. a Basal beating-rate prior to substance application. b Beating-rate of the first 30 min after substance application. c 0.5–6 h time course of beating-rate in Cor.4U^® cells after substance application. d Area under the curve for the 6 h beating-rate measurement. Data represent mean values ± SEM, n = 4, *p < 0.05 vs. basal. Regular insulin Ins, isoproterenol iso, insulin glargine IGla, active metabolite of insulin glargine IGlaM1, insulin degludec IDeg