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Table 1 Glucose consumption and lactate release over 24 h, and ratio between lactate production and glucose consumption in human vascular smooth muscle cells in culture

From: Inflammation, glucose, and vascular cell damage: the role of the pentose phosphate pathway

(a) Control
  Basal 10 ng/mL IL-1β 0.5 mmol/L sodium azide
Initial extracellular glucose (mmol/L) Glucose consumption (pmol per cell) Lactate production (pmol per cell) Lactate: glucose ratio Glucose consumption (pmol per cell) Lactate production (pmol per cell) Lactate: glucose ratio Glucose consumption (pmol per cell) Lactate production (pmol per cell) Lactate: glucose ratio
5.5 5.3 ± 0.3 7.0 ± 0.6 1.3 ± 0.1 6.0 ± 0.3* 7.1 ± 0.6 1.2 ± 0.1 6.9 ± 0.1* 8.8 ± 0.9 1.3 ± 0.1
11 4.9 ± 0.6 6.4 ± 1.2 1.3 ± 0.2 8.1 ± 0.9* 9.7 ± 1.7 1.2 ± 0.1    
22 5.5 ± 0.5 7.6 ± 0.8 1.4 ± 0.1 10.0 ± 0.9* 13.0 ± 1.6* 1.3 ± 0.1 13.2 ± 0.8* 17.7 ± 2.2* 1.4 ± 0.2
  1. Results are expressed as the mean ± standard error of 5–28 separate experiments
  2. EV cells with a lentiviral empty vector, GLUT1+ cells with a lentiviral vector containing human GLUT1 cDNA
  3. *P < 0.05 vs the respective control. P < 0.05 vs the respective value in 5.5 mmol/L glucose
(b) EV and GLUT1+
Initial extracellular glucose (mmol/L) Glucose consumption Lactate production Lactate:glucose ratio
  EV GLUT1+ EV GLUT1+ EV GLUT1+
5.5 6.5 ± 0.7 6.0 ± 0.1 7.9 ± 0.4 6.7 ± 0.5 1.2 ± 0.1 1.1 ± 0.1
22 6.5 ± 0.4 6.2 ± 0.2 9.3 ± 1.0 8.6 ± 0.3 1.5 ± 0.1 1.4 ± 0.2
  1. Results are expressed as the mean ± standard error of 5–28 separate experiments
  2. EV cells with a lentiviral empty vector, GLUT1+ cells with a lentiviral vector containing human GLUT1 cDNA
  3. *P < 0.05 vs the respective control. P < 0.05 vs the respective value in 5.5 mmol/L glucose