|
(a) Control
|
|---|
| |
Basal
|
10 ng/mL IL-1β
|
0.5 mmol/L sodium azide
|
|---|
|
Initial extracellular glucose (mmol/L)
|
Glucose consumption (pmol per cell)
|
Lactate production (pmol per cell)
|
Lactate: glucose ratio
|
Glucose consumption (pmol per cell)
|
Lactate production (pmol per cell)
|
Lactate: glucose ratio
|
Glucose consumption (pmol per cell)
|
Lactate production (pmol per cell)
|
Lactate: glucose ratio
|
|---|
|
5.5
|
5.3 ± 0.3
|
7.0 ± 0.6
|
1.3 ± 0.1
|
6.0 ± 0.3*
|
7.1 ± 0.6
|
1.2 ± 0.1
|
6.9 ± 0.1*
|
8.8 ± 0.9
|
1.3 ± 0.1
|
|
11
|
4.9 ± 0.6
|
6.4 ± 1.2
|
1.3 ± 0.2
|
8.1 ± 0.9*†
|
9.7 ± 1.7†
|
1.2 ± 0.1
| | | |
|
22
|
5.5 ± 0.5
|
7.6 ± 0.8
|
1.4 ± 0.1
|
10.0 ± 0.9*†
|
13.0 ± 1.6*†
|
1.3 ± 0.1
|
13.2 ± 0.8*†
|
17.7 ± 2.2*†
|
1.4 ± 0.2
|
|
(b) EV and GLUT1+
|
|---|
|
Initial extracellular glucose (mmol/L)
|
Glucose consumption
|
Lactate production
|
Lactate:glucose ratio
|
|---|
| |
EV
|
GLUT1+
|
EV
|
GLUT1+
|
EV
|
GLUT1+
|
|---|
|
5.5
|
6.5 ± 0.7
|
6.0 ± 0.1
|
7.9 ± 0.4
|
6.7 ± 0.5
|
1.2 ± 0.1
|
1.1 ± 0.1
|
|
22
|
6.5 ± 0.4
|
6.2 ± 0.2
|
9.3 ± 1.0
|
8.6 ± 0.3
|
1.5 ± 0.1
|
1.4 ± 0.2
|
- Results are expressed as the mean ± standard error of 5–28 separate experiments
-
EV cells with a lentiviral empty vector, GLUT1+ cells with a lentiviral vector containing human GLUT1 cDNA
- *P < 0.05 vs the respective control. †
P < 0.05 vs the respective value in 5.5 mmol/L glucose
