Fig. 7

Endothelial dysfunction evoked by IL1β in rat mesenteric microvessels. (a) Effect of IL1β (2.5 ng/mL) on the endothelium-dependent relaxations induced by acetylcholine (ACh, 0.1 nmol/L to 10 µmol/L) in isolated mesenteric arteries from Sprague–Dawley (SD) rats incubated with medium containing 5.5 or 22 mmol/L glucose. Data are expressed (mean ± SE) as the percentage of the previous contraction induced with 1 µmol/L noradrenaline (NA), which averaged 9.36 ± 0.77, 9.33 ± 0.58, 9.04 ± 0.83, and 9.67 ± 0.74 mNewtons, respectively. The number of segments used for every curve, which were obtained from 12 animals are in parenthesis. *P < 0.05 vs respective control. ^† P < 0.05 vs respective value in 5.5 mmol/L glucose. (b) Effect of 6-ANAM (2 mmol/L) on the endothelium impairment induced by IL1β in rat vascular mesenteric segments incubated in 5.5 mmol/L glucose. Data are expressed (mean ± SE) as the percentage of the previous contraction induced with NA, which averaged 10.67 ± 0.76, 10.10 ± 0.84, 12.10 ± 1.37, and 10.83 ± 1.14 mNewtons, respectively. The number of segments used for every curve, which were obtained from 5 animals are in parenthesis. *P < 0.05 vs control. (c) Effect of 6-ANAM on the endothelium impairment induced by IL1β in rat vascular mesenteric segments incubated in 22 mmol/L glucose. Data are expressed (mean ± SE) as the percentage of the previous contraction induced with NA, which averaged 10.07 ± 0.55, 10.30 ± 0.71, 9.27 ± 1.76, and 10.87 ± 1.15 mNewtons, respectively. The number of segments used for every curve, which were obtained from 5 animals are in parenthesis. *P < 0.05 vs control. ^† P < 0.05 vs IL1β