Fig. 3

IL1β modifies glucose metabolic profile, activating the pentose phosphate pathway (PPP). (a) Cells were treated for 18 h with IL1β (10 ng/mL) in medium containing 5.5 or 22 mmol/L glucose and the ¹⁴CO₂ production was measured from [6-¹⁴C]glucose. Results are the mean ± standard error of 8–12 separate experiments. b Cells were treated for 18 h with IL1β (10 ng/mL), in medium containing 5.5 or 22 mmol/L glucose. The PPP flux was then measured for 120 min by assessing the difference between ¹⁴CO₂ production from [1-¹⁴C]glucose—and that of [6-¹⁴C]glucose. Results are the mean ± standard error of 8–12 separate experiments. (c) G6PD levels, determined by Western blot, in cells treated for 18 h with IL1β (10 ng/mL), in medium containing 5.5 or 22 mmol/L glucose. Results are the mean ± standard error of 5 separate experiments, expressed as percentage of the expression produced by treatment with IL1β in medium containing 5.5 mmol/L glucose. (d) Total glutathione and GSH production in cells exposed for 18 h to IL1β (10 ng/mL) in medium containing 5.5 or 22 mmol/L glucose. Results are the mean ± standard error of 3–5 separate experiments. *P < 0.05 vs respective control (c). ^† P < 0.05 vs respective value in 5.5 mmol/L glucose