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Fig. 1 | Cardiovascular Diabetology

Fig. 1

From: Inflammation, glucose, and vascular cell damage: the role of the pentose phosphate pathway

Fig. 1

Inflammation, but not exposure to high extracellular glucose, enhances consumption of glucose. a Cytochalasine-B sensitive uptake of 3H-deoxyglucose by cells previously treated for 18 h with 10 ng/mL IL1β in medium containing 5.5 or 22 mmol/L glucose. Results are the mean ± standard error of 13–15 separate experiments. *P < 0.05 vs the respective control (c). b GLUT1 levels, determined by Western blot, in cells exposed for 18 h to IL1β (10 ng/mL) in medium initially containing 5.5 or 22 mmol/L glucose. The gels and blots shown are representative of 4–5 separate experiments. Data are expressed as percentage of the basal expression in cells in medium initially containing 5.5 mmol/L glucose *P < 0.05 vs the respective control (c). c Cytokine-induced consumption of glucose in cells exposed for 24 to different concentrations of IL1β (1–10 ng/mL). Data were calculated by subtracting at every point the basal consumption of glucose. Results are expressed as mean ± standard error of 8–11 separate experiments. *P < 0.05 vs 5.5 mmol/L glucose. P < 0.05 vs 11 mmol/L glucose. d Uptake of 3H-deoxyglucose by cells previously treated for 18 h with different concentrations of IL1β (1–10 ng/mL) in medium containing 5.5 mmol/L glucose in the presence or absence of the glucose transporter inhibitor cytochalasin B (20 µmol/L). Results are the mean ± standard error of 3 separate experiments. *P < 0.05 vs cytochalasin B-treated cells

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