Fig. 4

Knock-down of miR-185 under high and oscillating glucose. a q-PCR of miR-185 in HUVECs cultured in OG, HG and NG, following transfection with inhibitor of miR-185. Data are means (± SEM, **p < 0.01, ***p < 0.001 vs control;^###p < 0.001 vs OG vs OG+, and HG vs HG+; ^†††p < 0.001 OG vs HG on the left bars). b Number of viable cells in cell suspensions with anti-miR-185 inhibitor. c Western blot analysis of GPx-1 with or without anti-miR-185 during NG, OG and HG. d GPx activity in HUVECs during miR-185 inhibition in scrambled sample during NG, OG and HG. The results are expressed as mean (±SEM), (*p < 0.05, ***p < 0.001 vs control). Symbols over the bars refer to differences between the conditions shown under the bars (^§§§p < 0.01)