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Fig. 3 | Cardiovascular Diabetology

Fig. 3

From: Oscillating glucose induces microRNA-185 and impairs an efficient antioxidant response in human endothelial cells

Fig. 3

Oscillating glucose-induced miR-185 modulates cellular antioxidant response to oxidative stress. a Mean expression values of intracellular miR-185 in OG, HG and NG. **p < 0.01. b Sequence alignment between miR-185 and its 2 putative binding sites in the 3′-UTR of GPx-1 mRNA from humans, with sequences recognized by miR-185 seed sequence shown in bold. c Functional binding assay. The binding of miRNA to the 3′UTR results in repression of luciferase gene expression. Firefly luciferase values were normalized to protein content. Data are expressed as relative light units (RLU) and in relation to empty vector. Mean values (±SEM) of three independent experiments are shown. *p < 0.05 vs pMIR,#p < 0.05 pMIR-GPx1 plus mimic vs pMIR plus mimic

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