Fig. 8

Circulating regulatory populations in diabetic patients and healthy controls. The number of Tregs (CD4⁺CD25⁺CD127⁻) T cells (a), FoxP3^high Tregs (CD4⁺CD25⁺ CD127⁻) T cells (b), CTLA4^high Tregs (CD4⁺CD25⁺ CD127⁻FoxP3^high) T cells (c) and FoxP3 MFI on Tregs (CD4⁺CD25⁺ CD127⁻) T cells (d). Representative dot plot analysis of Treg sub-populations (CD3⁺CD4⁺CD25⁺CD127⁻; CD3⁺CD4⁺CD25⁺CD127⁻FoxP3⁺; CD3⁺CD4⁺CD25⁺CD127⁻FoxP3⁺CTLA4⁺) (e) that were first identified using the gating strategy from Figs. 1, 2 and Additional file 1: Figure S1. A total of 2 ml blood was analysed and the total number of each cell population was calculated as described in the “Methods” section. Each dot represents one individual and the horizontal line represents the mean value in each group. P values represent difference between groups assessed by t test. MFI mean fluorescence intensity