Fig. 8
From: Nicorandil attenuates carotid intimal hyperplasia after balloon catheter injury in diabetic rats
Effect of εPKC on VSMCs migration induced by high glucose. a Representative western blots for εPKC translocation in VSMCs with or without nicorandil or 5-HD. b Quantitative analysis of εPKC translocation. εPKC translocation was induced by high glucose. Nicorandil inhibited the increase of εPKC translocation by opening mitoKATP channel. c Representative western blots for εPKC protein in VSMCs with or without εPKC siRNA transfection. d Quantitative analysis of εPKC protein. εPKC protein in high-glucose-εPKC siRNA group was reduced to 24.94 ± 7.67 % of that in high-glucose-scramble siRNA group. e VSMCs migration was assessed by wound healing assay. f Quantitative analysis of percentage of migrated area in control group, high glucose + scramble siRNA group, and high glucose + εPKC siRNA group. εPKC siRNA knockdown prevented high glucose-induced cell migration. Bars represent mean ± SE. *p < 0.05, **p < 0.01