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Fig. 1 | Cardiovascular Diabetology

Fig. 1

From: Vascular endothelial microparticles-incorporated microRNAs are altered in patients with diabetes mellitus

Fig. 1

Circulating MPs characterization. a Circulating MPs were collected by 20,000g ultracentrifugation of platelet-deficient plasma. Fluorescent polystyrene particles (0.7–0.9, 2 and 6 µm) were used to evaluate the size of isolated MPs. In flow cytometer, analyzed MPs (red) had a size <1 μm as demonstrated using fluorescent polystyrene particles. b The obtained pellet after 20,000g ultracentrifugation was fixed in 3 % glutaraldehyde PBS overnight at 4 °C and embedded in Epon-pur. 50 nm sections were prepared on copper grids. Samples were visualized on a Philips CM 10 electronic microscope and analyzed with analySiS software (Olympus)

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Cardiovascular Diabetology

ISSN: 1475-2840