Fig. 6
From: Interleukin-1β mediates high glucose induced phenotypic transition in human aortic endothelial cells
The influence of blocking IL-1β treatment on the protein expressions of CD31, FSP1, a-SMA, and IL-1β. (a–f) HAECs were incubated for 48 h with anti-IL-1β antibodies (1000 ng/ml) in the presence of NG or HG. (a1–f1) We performed gene-silencing experiments using transfection with siRNA specific for IL-1β. The protein expressions of IL-1β, CD31, FSP1 and α-SMA were assessed by western blotting. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM). Anti-IL-1β: anti-IL-1β antibodies (1000 ng/ml). *P < 0.05 vs. NG or anti-IL-1β, # P < 0.05 vs. HG or HG +Vehicle