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Fig. 5 | Cardiovascular Diabetology

Fig. 5

From: Interleukin-1β mediates high glucose induced phenotypic transition in human aortic endothelial cells

Fig. 5

The influence of high glucose or IL-1β on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1β alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1β in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1β (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1β (10 ng/ml) treatment for 48 h. Scale bar, 75 μm

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