Fig. 4From: Interleukin-1β mediates high glucose induced phenotypic transition in human aortic endothelial cellsEffect of high glucose and IL-1β alone or in combination on the protein expressions of CD31, FSP1 and α-SMA in HAECs. a–d HAECs were incubated for 48 h with NG and HG. Mannitol was used as a control for hyperosmolarity. Representative western blots (a) and quantitative determinations of CD31, FSP1 and α-SMA protein levels (b–d) are presented. e–h HAECs were treated for 48 h with NG, HG, IL-1β (10 ng/ml)and HG in the presence of the IL-1β (10 ng/ml). Representative western blots (E) and quantitative determinations of CD31, FSP1 and α-SMA protein levels (f–h) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), MN 5.5 mM glucose + 24.5 mM mannitol, IL-1β (10 ng/ml), HG + IL-1β: high glucose (30 mM) + IL-1β (10 ng/ml) *P < 0.05 vs. MN or NG, **P < 0.01 vs. NG, # P < 0.05 vs. HGBack to article page