Fig. 3
From: Interleukin-1β mediates high glucose induced phenotypic transition in human aortic endothelial cells
Effects of PKCβ on high glucose induced IL-1β up-regulation. Confluent cultures of HAECs were exposed to NG, HG, PMA (30 nM) and HG in the presence of the selective PKCβ inhibitors (LY317615, 0.3 μM) for 48 h. Real-time PCR analyses showed mRNA expression of PKCβ and IL-1β (a, b). Representative western blots (c) and quantitative determinations of PKCβ and IL-1β (d, e) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), PMA (30 nM): phorbol 12-myristate13-acetate; LY (0.3 uM): LY317615; *P < 0.05 vs.NG, **P < 0.01 vs. NG, # P < 0.05 vs. HG or PMA