Fig. 2
From: Interleukin-1β mediates high glucose induced phenotypic transition in human aortic endothelial cells
Effects of high glucose on PKCβ mRNA and protein expression in HAECs. a, c–e HAECs were incubated for 48 h with increasing concentrations of glucose (5.5, 15, 30 mM). Mannitol was used as a control for hyperosmolarity. b, d–f HAECs were grown in a 30 mM glucose medium for 12–48 h. PKCβ mRNA level (a, b) was assayed by quantitative RT-PCR. Representative western blots (c, d) and quantitative determinations of PKCβ protein levels (e, f) are presented expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose 30 mM), MN 5.5 mM glucose + 24.5 mM mannitol. *P < 0.05 vs. MN or NG