Fig. 1
From: Interleukin-1β mediates high glucose induced phenotypic transition in human aortic endothelial cells
Effect of high glucose on IL-1β mRNA and protein expression in HAECs. a, b HAECs were incubated for 48 h with increasing concentrations of glucose (5.5, 15, 30 mM). Mannitol was used as a control for hyperosmolarity. c, d HAECs were grown in a 30 mM glucose medium for 12–48 h. IL-1β protein level was measured in the supernatant using ELISA and IL-1β mRNA level was assayed by quantitative RT-PCR. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), MN 5.5 mM glucose + 24.5 mM mannitol. *P < 0.05 vs. MN or NG, **P < 0.01 vs. MN or NG