Fig. 1

Immunoblots for iNOS in VSMCs from control (a) and STZ-diabetic rats (b) in the presence of endogenous adenosine modulators. VSMCs were incubated with cytomix comprising 10 ng/mL interleukin (IL)-1β, 10 ng/mL interferon (IFN)-γ and 25 ng/mL tumor necrosis factor (TNF)-α plus 1 µg/mL LPS for 24 h. Representative blots are shown. Densitometric analysis of iNOS protein levels are shown as % cytomix. The data are presented as mean ± SEM (n = 8). *P < 0.05, **P < 0.01 (two-tailed t-test). ADA, adenosine deaminase; 8-PT, 8-phenyltheophylline