Table 1 Quantitative summary of the different cellular structures in the remote, border and infarct zone in a 4-week telmisartan-treated diabetic mouse (* p < 0.05)
Cardiomyocyte ultrastructure score by tissue electron microscopy | ||||
|---|---|---|---|---|
Cell structure | Characteristic feature | Remote | Border | Infract |
Mean ± SD | Mean ± SD | Mean ± SD | ||
Nuclei | Notched/furrowed membrane | 4.5 ± 0.7 | 3.9 ± 1.0 | 1.1 ± 0.4 |
Homogenous chromatin granules | 4.8 ± 0.4 | 4.3 ± 0.8 | 1.3 ± 0.5 | |
Chromatin accumulated along nuclear membrane | 5.0 ± 0.0 | 4.2 ± 0.8 | 1.9 ± 0.6 | |
Chromatin clots within nucleus | 4.8 ± 0.4 | 4.3 ± 0.8 | 1.3 ± 0.5 | |
Dense chromatin | 4.9 ± 0.3 | 4.3 ± 0.9 | 1.3 ± 0.5 | |
Dark chromatin finely structured | 4.9 ± 0.3 | 4.5 ± 0.7 | 1.2 ± 0.4 | |
Mitochondria | Dense peri-nuclear accumulation | 4.6 ± 0.5 | 3.8 ± 1.4 | 1.2 ± 0.4 |
Fine filaments/glycogen granules between nucleus & mitochondria | 4.8 ± 0.4 | 4.1 ± 1.0 | 1.3 ± 0.5 | |
Destroyed cristea | 4.8 ± 0.4 | 3.7 ± 1.0 | 1.1 ± 0.4 | |
Few mitochondria near nucleus | 4.8 ± 0.4 | 3.9 ± 1.5 | 1.1 ± 0.3 | |
Mitochondria isolated in niche | 4.7 ± 0.6 | 4.1 ± 1.4 | 1.1 ± 0.4 | |
Myofibrils | Myofibrils aligned in one row | 4.8 ± 0.5 | 3.9 ± 0.8 | 1.0 ± 0.2 |
T-tubules contain basal lamina | 4.9 ± 0.4 | 4.7 ± 0.6 | 1.1 ± 0.3 | |
Myofibrils are contracted | 4.8 ± 0.4 | 4.2 ± 0.9 | 1.0 ± 0.3 | |
Z-line disruption | 4.9 ± 0.3 | 4.1 ± 0.7 | 1.0 ± 0.2 | |
Lipid droplets between ruptured myofibrils | 4.3 ± 0.5 | 3.6 ± 1.1 | 1.2 ± 0.4 | |
Collagen | 4.0 ± 0.9 | 3.1 ± 0.9 | 1.0 ± 0.2 | |
Overall TEM scores | 4.7 ± 0.3 | 3.1 × 0.4* | 1.2 × 0.2 | |