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Fig. 2 | Cardiovascular Diabetology

Fig. 2

From: Metformin improves the angiogenic potential of human CD34+ cells co-incident with downregulating CXCL10 and TIMP1 gene expression and increasing VEGFA under hyperglycemia and hypoxia within a therapeutic window for myocardial infarction

Fig. 2

Effect of CM from CD34+ cells exposed to different conditions on in vitro angiogenic assays. a HUVEC were plated on Matrigel with CM from 2.0 × 105 CD34+ cells treated with euglycemia, euglycemia and hypoxia, hyperglycemia or hyperglycemia and hypoxia in the presence and absence of metformin. EBM-2 medium containing the VEGF inhibitor sunitinib (14 µmol/L) was used as a negative control for the assay. The highlighted image shows the greatest tube length, which was achieved in HUVEC incubated with CM-derived from CD34+ cells treated with hyperglycemia and hypoxia in the presence of metformin. b Tube length at 6 h was expressed as a percentage of the tube length of HUVEC treated with 5.5 mmol/L glucose CM (n = 3); *P < 0.05 compared pairwise, i.e., condition with metformin versus metformin-untreated condition

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Cardiovascular Diabetology

ISSN: 1475-2840