Fig. 2

Polydatin regulated LDLR and GCK expression possibly through PCSK9. a Co-IP assay was performed to explore whether polydatin affected the combination of PCSK9 and LDLR according to the “Methods” above. b The interfering efficiency of PCSK9 by siRNA was screened, and the second sequence si1153 was chosen for subsequent experiments and the negative sequences were used as control. c, d LDLR and GCK expression under PCSK9 knockdown conditions. e, f LDLR and GCK expression under PCSK9 overexpression conditions. Overexpression of PCSK9 with Human wild-type PCSK9 plasmids and the vector PENTER plasmid were used as control. SiRNA and overexpression experiments were all divided into three groups: Control group, IR group, and IR + polydatin group. The first three lanes were treated with the negative SiRNA squences or pENTER vector as control and the last three lanes were treated with siRNA-1153 sequences or Human wild-type PCSK9 plasmids, respectively. ^### p < 0.001 vs. control group (Con); *p < 0.05, **p < 0.01 and ***p < 0.001 vs. LDLR levels in the control group (Con); ^{^^} p < 0.01 and ^{^^^} p < 0.001 vs. PCSK9 levels in the control group (Con)