Fig. 7
From: Insulin treatment increases myocardial ceramide accumulation and disrupts cardiometabolic function
Chronic insulin injections disrupt myocardial mitochondrial function. 16-week-old male mice received injections of PBS (daily), insulin (INS; daily; 0.75 mg/kg), myriocin (MYR, thrice weekly; 3 mg/kg), or INS + MYR. Mitochondrial assessments were determined in permeabilized (saponin, 50 µg/ml) myocardium. To measure mitochondrial respiration (a), samples were treated with: GM L , Glutamate (10 mM) + Malate (2 mM); GM D , + ADP (2.5 mM); GMS D , + Succinate (10 mM). Respiratory control ratio (RCR; b) and Complex II Factor (c) were determined by the analysis indicated. *P < 0.05 for condition vs. PBS