Fig. 6

Insulin injection increases heart ceramides. 16-week-old male mice received injections of PBS (daily), insulin (INS; daily; 0.75 mg/kg), myriocin (MYR, thrice weekly; 3 mg/kg), or INS + MYR. INS treatment increased myocardial ceramide accrual (a) and SPT2 (b). Serum adiponectin was also measured (c). *P < 0.05 for condition vs. PBS. ^#P < 0.05 for INS + MYR vs. INS alone