Table 1 Gene-specific primer sequences used for quantitative real-time PCR
From: Early impairment of coronary microvascular perfusion capacity in rats on a high fat diet
Gene | Forward primer | Reverse primer |
|---|---|---|
α-Skeletal actin (αSKA) | TGAGACCACCTACAACAGCA | CCAGAGCTGTGATCTCCTTC |
Angiopoietin-like 4 (Angptl4) | CCTTTCCCTGCCCTTCTCTAC | AGGCTCTTGGCACAGTTAAGG |
Atrial natriuretic factor (ANF) | AGCGAGCAGACCGATGAAGC | GCAGAGTGGGAGAGGTAAGGC |
Cluster of differentiation 68 (CD68) | TAATACGACTCACTATAGGG | TAGAAGGCACAGTCGAGG |
Cyclophilin-A (Cyclo) | CAAATGCTGGACCAAACACAA | TTCACCTTCCCAAAGACCACAT |
Endothelial nitric oxide synthase (eNOS) | TGCTGCCCGAGATATCTTCAGT | GGCTGCCTTTTTCCAGTTGTTC |
Intercellular adhesion molecule (ICAM) | AAACGGGAGATGAATGGTACCTAC | TGCACGTCCCTGGTGATACTC |
Monocyte chemoattractant protein-1 (MCP1) | ATGCAGTTAATGCCCCACTC | TGCTGCTGGTGATTCTCTTG |
Pyruvate dehydrogenase kinase 4 (PDK4) | GCATTTCTACTCGGATGCTCATG | CCAATGTGGCTTGGGTTTCC |
Vascular adhesion molecule (VCAM) | GGCTCGTACACCATCCGC | CGGTTTTCGATTCACACTCGT |