Inhibition of high glucose-increased ALP mRNA expression and activity by ROCK inhibitors. a Inhibition of high glucose-induced increases in ALP mRNA levels in HCSMCs by fasudil and Y-27632. HCASMCs were cultured in high glucose-containing media for 5 days. The values represent the mean ± SEM (n = 3). b, c Inhibition of high glucose-induced increases in ALP activity of HCSMCs by fasudil and Y-27632. HCASMCs were cultured for 10 days. Representative images of the ALP-staining (b) and the percentages of the ALP-positive area relative to the total surface area of HCASMCs (c) are shown. The data are presented as mean ± SEM (n = 5). †
P < 0.01.