Skip to main content
Fig. 2 | Cardiovascular Diabetology

Fig. 2

From: Partial inhibition of the ubiquitin–proteasome system ameliorates cardiac dysfunction following ischemia–reperfusion in the presence of high glucose

Fig. 2

Lactacystin and MG-132 administration attenuate proteasomal activities during ischemia–reperfusion. Isolated rat hearts were perfused under high glucose (33 mM) conditions vs. controls (11 mM) and subjected to global ischemia and reperfusion as earlier described. For UPS inhibition, either 5 μM lactacystin or 10 μM MG-132 was added for the first 20 min of the reperfusion period. The respective proteasomal activities evaluated: a, d trypsin-like (LSTR), b, e chymotrypsin-like (LLVY), c, f caspase-like (LLE). Values are expressed as mean ± SEM (n = 8). *p < 0.05, **p < 0.01, ***p < 0.001 vs. respective controls.

Back to article page

Cardiovascular Diabetology

ISSN: 1475-2840