Skip to main content
Fig. 10 | Cardiovascular Diabetology

Fig. 10

From: Partial inhibition of the ubiquitin–proteasome system ameliorates cardiac dysfunction following ischemia–reperfusion in the presence of high glucose

Fig. 10

Increased expression of autophagy markers following UPS inhibition with ischemia–reperfusion. Isolated rat hearts were perfused under simulated acute hyperglycemia conditions (33 mM glucose) vs. controls (11 mM glucose) for 60 min and thereafter subjected to 20 min of global ischemia, followed by 60 min of reperfusion. 5 μM lactacystin was added for the first 20 min of reperfusion. Western blot analysis for: a LC3-II and b p62 protein expression levels are shown with β-actin as loading control. Values are expressed as mean ± SEM (n ≥ 4). *p < 0.05, ***p < 0.001 vs. respective controls.

Back to article page

Cardiovascular Diabetology

ISSN: 1475-2840