Non-targeted cardiac metabolomics of MuRF2−/− mice after 26 weeks challenge with high fat diet. a Principal components analysis, b Partial least squares-discriminant analysis (PLS-DA) and variable importance in the projection analysis, c Heat map of metabolites identified by non-targeted GC/MS analysis of cardiac tissue. d Enrichment by pathway-associated metabolite sets and e location-Based Metabolite sets determined from VIP significant and t test significant metabolites identified. N = 3/group. Significance determined as p < 0.05.