MuRF2 alters nuclear localization of PPARγ1 in a ubiquitin ligase region (RING)-dependent manner without altering steady state protein levels (as found in vivo). Increased MuRF2 alters PPARγ1 localization from primarily nuclear a to increased cytosolic/perinuclear localization b in HL-1 cardiomyocyte-derived cells. MuRF2 lacking ubiquitin ligase activity (∆RING FingerMuRF2, c does not alter PPARγ1 localization compared controls (top row), indicating MuRF2’s dependence on its ubiquitin ligase activity in mediating these changes. Representative of three biological replicates. Right Percentages based on the number of cells analyzed (N = 58, 68, and 88 in FLAG-PPARγ1, FLAG-PPARγ1 + HA-MuRF2, and FLAG-PPARγ1 + HA-∆RING-MuRF2 groups, respectively).