Analysis of tissue triglyceride, glycogen, and fat mass in MuRF2−/− mice after high fat diet challenge. a Triglyceride analysis of cardiac left ventricle (LV), liver, and skeletal muscle (gastrocnemius). b Glycogen analysis of cardiac LV, liver, and skeletal muscle (gastrocnemius). c Magnetic resonance imaging (MRI) analysis of fat mass, lean body mass, and free water at baseline, 6, 12, and 22 weeks HFD. Values expressed as Mean ± SE. A one-way ANOVA was performed to determine significance of cardiac LV triglyceride and glycogen concentrations, followed by a Holm-Sidak pairwise comparison to determine significance between groups. A Student’s t test was performed comparing MuRF2−/− vs. MuRF2+/+ groups in all other studies. Numbers above bars represent number of animals (N) included in each experiment (N = MuRF2+/+, MuRF2−/− in c). *p < 0.001, **p < 0.01, #p < 0.05.