High fat diet-induced increases in PPAR-regulated gene (mRNA) levels are exaggerated in cardiac MuRF2−/− hearts. RT-qPCR analysis of cardiac mRNA of genes identified as PPAR isoform “specific” based on cardiac transgenic PPARα, PPARβ, and PPARγ1 studies as described in the text. a Cardiac PPARα target gene expression, b PPARβ-regulated mRNA target genes involved in glucose metabolism, c PPARβ-regulated mRNA target genes involved in fatty acid metabolism. d PPARγ1-regulated mRNA target genes. Values expressed as Mean ± SE. Values expressed as Mean ± SE. The significance of observed differences in grouped mean values was determined using a One Way ANOVA followed by Holm-Sidak pairwise post hoc analysis. N per group indicated above graph. *p ≤ 0.001, **p < 0.01, #p < 0.05.