Role of MuRF2 in regulating PPAR isoform activity and its role in high fat diet cardiac hypertrophy in vivo. Isolation of cardiac nuclei from MuRF2−/− and sibling wild type mouse hearts revealed increases in a PPAR∝, PPARβ/δ, and PPARγ DNA binding activity using PPRE-DNA as bait and ELISA detection of PPARα protein (N = 4/group). b Experimental design of high fat diet (60%)-induced cardiomyopathy. c High fat diet induces cardiac MuRF2 levels after 26 weeks HFD (N = 3/group). d Endogenous MuRF2 inhibits HFD-induced LV Mass and heart wet weights, as MuRF2−/− hearts have a significant increase in heart weight normalized to body weight and tibia length (N = 5/group). e Endogenous MuRF2, found in skeletal muscle and the heart does not affect overall body weight (N indicated below graph). Values expressed as Mean ± SE. Statistical analysis was performed using a Student’s t-test comparing MuRF2−/− and MuRF2+/+ groups. *p ≤ 0.001, **p < 0.01. §p < 0.05 by One Way ANOVA, ¶p < 0.05 vs. MuRF2+/+ baseline by multiple comparisons. $p < 0.05 vs. time-matched MuRF2+/+ (Student’s t-test).