Table 9 qRT-PCR results
Diabetes Vehicle vs. Control Vehicle | ||||||||
|---|---|---|---|---|---|---|---|---|
Description | Gene symbol | Acc. Number | DNA MICROARRAY | qRT-PCR | confirmed | |||
fold change | p value | log2 ratio (SD) | fold change | regulation | ||||
metallothionein 1a | Mt1a | NM_138826 | 2.49 | 0.005 | 2.36 (1.35) | 5.13 | up | yes |
metallothionein 2a | Mt2a | NM_001137564 | 3.06 | 0.003 | 2.36 (0.93) | 5.12 | up | yes |
cytochrome P450, family 26, subfamily b, polypeptide 1 | Cyp26b2 | NM_181087 | 3.08 | 0.003 | 2.50 (0.85) | 5.65 | up | yes |
galanin/GMAP prepropeptide | Gal | NM_033237 | 5.31 | 0.018 | 2.69 (0.86) | 6.43 | up | yes |
connector enhancer of kinase suppressor of Ras 1 | Cnksr2 | NM_001039011 | 2.48 | 0.025 | 0.002 (0.26) | 1.00 | no change | no |
Diabetes MVT vs. Diabetes Vehicle | ||||||||
Description | Gene symbol | Acc. Number | DNA MICROARRAY | qRT-PCR | confirmed | |||
fold change | p value | log2 ratio (SD) | fold change | regulation | ||||
metallothionein 1a | Mt1a | NM_138826 | −2.26 | 0.017 | −1,44 (0.91) | −2.71 | down | yes |
metallothionein 2a | Mt2a | NM_001137564 | −3.24 | 0.018 | −2.53 (0.68) | −5.76 | down | yes |
cytochrome P450, family 26, subfamily b, polypeptide 1 | Cyp26b2 | NM_181087 | −3.20 | 0.007 | −2.91 (0.32) | −7.50 | down | yes |
galanin/GMAP prepropeptide | Gal | NM_033237 | −7.96 | 0.007 | −2.72 (0.64) | −6.60 | down | yes |
connector enhancer of kinase suppressor of Ras 1 | Cnksr2 | NM_001039011 | −3.13 | 0.004 | −0.11 (0.33) | 1.08 | no change | no |