Fig. 3From: Chronic NF-κB blockade improves renal angiotensin II type 1 receptor functions and reduces blood pressure in Zucker diabetic ratsEffects of PDTC on Nox-2 and iNOS expression in the renal cortex of LZ and ZDF rats. mRNA level of Nox-2 (a) and iNOS (b) in the renal cortex was measured using qRT-PCR and normalized to GAPDH expression. c Protein expression of Nox-2 and iNOS in the renal cortex of LZ and ZDF rats was measured by western blot, and data were normalized using GAPDH expression. d The activity of NADPH oxidase in renal cortical homogenates was measured by using lucigenin-enhanced chemiluminescence and expressed as percentage of relative luminescence units (RLU)/μg protein. *P <0.05 vs LZ control; # P < 0.05 vs ZDF control (n = 5)Back to article page