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Fig. 2 | Cardiovascular Diabetology

Fig. 2

From: Cardiospecific CD36 suppression by lentivirus-mediated RNA interference prevents cardiac hypertrophy and systolic dysfunction in high-fat-diet induced obese mice

Fig. 2

Effect of RNAi in the setting of HFD feeding on CD36 expression. a Quantitative real-time RT-PCR for cardiac CD36 mRNA expression. Lentivirus mediated CD36 RNAi using short hairpin RNAs (shRNAs) lead to the downregulation of CD36. Mice in groups treated with sh-CD36 (N-CD36 and O-CD36) showed decreased CD36 mRNA expression by nearly 50 % in comparison with groups treated with sh-GFP (N-mock and O-mock). b Western blot analysis of heart tissue lysates from each group. GAPDH was introduced as an internal reference. Values are mean ± SD, N = 3 for each group. a, vs. N-mock; b, vs. N-CD36; c, vs. O-mock; (*) P < 0.05

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