Skip to main content

Table 2 Impact of LPS on inflammatory gene expression in WT and iNOS KO mice

From: Tissue inflammation and nitric oxide-mediated alterations in cardiovascular function are major determinants of endotoxin-induced insulin resistance

Group Heart Gastrocnemius
Interleukin-6 gene expression
WT + SAL 1.5 ± 0.9 1.4 ± 0.8
WT + LPS 9.6 ± 3.2p = 0.07 61 ± 26*
iNOS KO + SAL 4.1 ± 2.3 1.4 ± 0.6
iNOS KO + LPS 19.6 ± 4.7** 9.5 ± 0.9***
Monocyte Chemoattractant Protein-1 (MCP)-1 gene expression
WT + SAL 1.1 ± 0.4 1.1 ± 0.3
WT + LPS 2.6 ± 0.1* 4.9 ± 2.1
iNOS KO + SAL 1.8 ± 0.7 0.6 ± 0.6
iNOS KO + LPS 6.1 ± 2.1 p = 0.07 12.1 ± 5.3**
  1. Relative expression (Rq) of Interleukin-6 and Monocyte Chemoattractant Protein-1 (MCP-1) gene measured in tissues excised at the end of a hyperinsulinemic-euglycemic clamp. Data are Rq values relative to the control group (WT + SAL). Data are mean ± SEM. *p < 0.05 vs. WT + SAL; **p < 0.05 vs. iNOS KO + SAL; ***p < 0.05 vs. WT + LPS. iNOS inducible nitric oxide synthase, KO knock-out, LPS lipopolysaccharide, SAL saline, WT wild-type