Fig. 8
Tissue-specific insulin-stimulated glucose uptake (Rd in μg∙g−1∙min−1) for LPS-treated wild-type (WT) and iNOS knock-out (KO) mice (a) (Group 2A). In vivo glucose uptake was assessed 25 min after a 5 μCi bolus of 14C-2-deoxyglucose at the end of a hyperinsulinemic-euglycemic clamp (n = 3–7). Insulin levels in plasma (ng/ml) before and after the clamp (b). Basal and insulin-stimulated ex vivo muscle 2-DG was determined in isolated soleus (Panel c, n = 3–6). Tissues were incubated in media with or without insulin (10 mU/ml) for 30 min, then transferred to the same media containing 2-deoxy-D-glucose, 2-[1,2-3H] deoxy-D-glucose (2-DG, 0.25 μCi/ml), and D-[1-14C] mannitol (0.16 μCi/ml). Data are mean ± SEM. * p < 0.05 vs. WT + SAL, †p < 0.05 vs. KO + SAL, § p < 0.05 vs. Basal for same group