Fig. 10

Effects of LPS on skeletal muscle insulin signaling in wild-type (WT) and iNOS knock-out (KO) mice (Group 2A). Western blotting was performed for gastrocnemius extract for phosphorylation (p) of Akt at Ser473 (a, c), and for glycogen synthase kinase (GSK)-3β phosphorylation at Ser9 residue (b, d). Chemiluminescence on 4–12 % SDS-PAGE gels was quantified for skeletal muscle. Insulin-induced protein phosphorylation was quantified as the ratio phosphorylation and total Akt and GSK-3β chemiluminescence in gastrocnemius extract after a 2 h hyperinsulinemic-euglycemic clamp. β-actin was used as a loading control. Data are arbitrary units (AU) expressed as mean ± SEM (n = 8). * p < 0.05 by two-way ANOVA