Figure 5

Inhibition of PKC promotes angiogenesis induced by PDGF-C, but not VEGF-A, in hyperglycemic endothelial cells. A, B: Photographs show tube formation in HUVECs exposed to 5.5 mM (Low) (A) or 30 mM (High) (B) glucose in the presence of Bis I (4 μΜ), PDGF-C (50 ng/mL), PDGF-C + Bis I, VEGF (50 ng/mL), VEGF + Bis I, and VEGF + Sulforaphane (5 μΜ). PBS treatment served as control (Control). Sulforaphane was used as a negative control for the assay. Scale bar = 300 μm. C: Total tube length was analyzed in the experiment of Figure 5A (left) and 5B (right). *P < 0.05, **P < 0.01 vs Control (VEGF(−), PDGF-C(−), Bis I(−), and Sulforaphane(−)) group. ##P < 0.01 vs VEGF(+), PDGF-C(−), Bis I (−), and Sulforaphane (−) group. †P < 0.05 vs VEGF(−), PDGF-C(+), Bis I (−), and Sulforaphane (−) group. n = 5 ~ 9 for each group. Data represent means ± standard error of the mean.