Figure 4

Inhibition of PKC potentiates intracellular signaling induced by PDGF-C in endothelial cells. A: HUVECs exposed to 5.5 mM (Low) or 30 mM (High) glucose were treated with PDGF-C (50 ng/mL) or PDGF-C + Bis I (4 μΜ) and analyzed for signaling molecules by Western blot analysis. B: Relative expression levels of pERK and pAkt in the experiment of Figure 4A. Results are shown as the ratio of pAkt to Akt densities, and pERK to ERK densities, respectively. *P < 0.05, **P < 0.01 vs PDGF-C (−), Bis I (−) group. #P < 0.05, ##P < 0.01 vs PDGF-C (+), Bis I (−) group. n = 4 for each group. Data represent means ± standard error of the mean.