Figure 2

Expression of PDGFR-α is downregulated in hyperglycemic endothelial cells. A: PDGFR-α, PDGFR-β, VEGFR2, PDGF-C, and VEGF-A mRNA levels in HUVECs or HMVECs treated with 5.5 mM (Low) or 30 mM (High) glucose were assessed by quantitative real-time PCR. *P < 0.05, **P < 0.01 vs Low glucose HUVECs (n = 6 for each group). #P < 0.05, ##P < 0.01 vs Low glucose HMVECs (n = 6 for each group). Data represent means ± standard error of the mean. B: Western blot analysis of PDGFR-α expression in HUVECs treated with 5.5 mM (Low) or 30 mM (High) glucose. Relative quantification data are also shown (right). **P < 0.01 vs Low glucose (n = 6 for each group). Data represent means ± standard error of the mean. C: Western blot analysis of PDGFR-α expression in HMVECs treated with 5.5 mM (Low) or 30 mM (High) glucose. Relative quantification data are also shown (right). ##P < 0.01 vs Low glucose (n = 8 for each group). Data represent means ± standard error of the mean.