Figure 4
IL-1β increases vascular NADPH oxidase activity. (A) NADPH oxidase activity in microvascular mesenteric preparations from control SD rats after 30 min stimulation with IL-1β (1, 2.5, and 10 μg/mL). Bars represents the mean ± SE of the relative light units (RLU) obtained in 3 different experiments using tissues from 6 animals. (B) NADPH oxidase activity in microvascular mesenteric preparations from control SD rats after 30 min stimulation with 2.5 μg/mL IL-1β, either alone and in the presence of 100 μg/mL anakinra or 10 μmol/L apocynin. Bars represent the mean ± SE of the relative light units (RLU) obtained in 3 experiments using tissues from 9 animals. *p < 0.05 vs basal; †p < 0.05 vs IL-1β.