Figure 5From: Dipeptidyl peptidase-4 inhibitor linagliptin attenuates neointima formation after vascular injuryBrdU assay using rat aortic smooth muscle cells. (A) After rat aortic smooth muscle cells were plated, the cells were incubated in DMEM containing 0.1% FBS with or without 10 nM linagliptin for 48 h. Subsequently, cells were stimulated with PDGF (25 ng/ml) for 24 h. (B) Cells were incubated in DMEM with 0.1% FBS for 24 h. Subsequently, they were stimulated with DPP-4 (50 ng/ml) with or without 10 nM linagliptin for 24 h. BrdU solution was added during the last 2 h, and cells were harvested for measurement of DNA synthesis using a microplate reader at 450-620 nm. Mean data are expressed as the ratio of the control cell proliferation. Unpaired t-tests were performed to calculate statistical significance. Data are mean ± SEM. **p <0.01 vs. control PDGF(+), ##p <0.01 vs. control PDGF(+), *p <0.05 vs. control.Back to article page