Figure 1From: Activation of AMP-activated protein kinase by metformin protects human coronary artery endothelial cells against diabetic lipoapoptosisActivation of AMPK protects human coronary artery endothelial cells from lipoapoptosis. Cells were incubated in the presence or absence of palmitate (125 μM), metformin (A: 100 μM-2 mM), (B-E: 500 μM), with or without AICAR (200 μM) or Compound C (10 μM). (A) Metformin protects cells from lipoapoptosis in a dose-dependent manner after 24 h, an effect that is mimicked by AICAR (B) and blocked by Compound C after 24 h of incubation (C). Cleaved caspase 3 (CC3) levels over time (D) and in the presence or absence of metformin, AICAR and Compound C after 24 h (E). The protective effect of metformin against palmitate-induced apoptosis is lost upon downregulation of AMPK by siRNA transfection (F). Bars represent mean ± SEM. * p < 0.05 vs. control; # p < 0.05 vs. palmitate; § p < 0.05 vs. metformin + palmitate with Kruskal-Wallis one-way analysis of variance on ranks. n =4-5.Back to article page