Immunization with AGE-LDL increased IL-4, FoxP3 and IL-10 levels in spleens from diabetic mice. (A) mRNA transcript ratios based on GAPDH expression are shown for each gene of interest for all 3 groups (n = 10 per group). (B) Secretion patterns of cytokines determined in supernatants derived from splenocytes stimulated with AGE-LDL among groups were analyzed with milliplex assay. White bars = controls, bars with stripes = Alum adjuvant and black bars = AGE-LDL + Alum adjuvant. *indicates p < 0.05 vs. control, **indicates p < 0.01 vs. control, and #indicates p < 0.05 vs. Alum.