Immunization with AGE-LDL and Alum adjuvant did not significantly change atherosclerotic lesion composition in the aortic root of diabetic apoE -/- and LDLR-/- mice. A and C, Representative photomicrographs showing Oil-red O (scale bar = 100 μm) and collagen (Masson), macrophage, and smooth muscle cell (SMA) stained aortic root sections (scale bar = 120 μm) from each group (n = 6 per group). B. Oil-red O stained area in the aortic root: white circles represent animals from the PBS-treated control group, black squares represent animals from the Alum adjuvant group, and white squares represent animals from the AGE-LDL + Alum treated group. *indicates p < 0.05 vs. control. D, Quantification of collagen, macrophages and smooth muscle cells from the 3 groups are shown: white bars represent animals from the control group, bars with stripes represent animals from the Alum adjuvant group, and black bars represent animals from AGE-LDL + Alum treated group.